Imagexpress Micro Confocal Filter User Manual

Posted : admin On 12/21/2021
Imagexpress Micro Confocal Filter User Manual
  1. User’s manual of the laser system you are using. (8) Shutter The shutter is for the incident laser beam. You may select full-open or close. For non-confocal observation, always keep the shutter closed. Red LED light on the operation panel of CSU-X1 will be OFF when the shutter is closed. (9) Interlock.
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  3. Run 3D cellular assays with confocal results – at a speed you’d only expect from widefield screening, with Molecular Devices’ new confocal HCS system Molecular Devices is delighted to introduce the ImageXpress® Micro Confocal High-Content Imaging System! This exciting new system allows you.

Molecular Devices Imagexpress Micro Conf…

Molecular Devices ImageXpress Micro Confocal

Explore new dimensions with the confocal system for complex biology. The ImageXpress® Micro Confocal High-Content Imaging System enables users to expand the boundaries of research with the ultimate combination of speed, sensitivity, and flexibility in a turn-key solution.

We have been awarded the NIH Shared Instrumentation Grant (S10OD026899) to upgrade our high-content automated cellular imaging and analysis machine to the Molecular DevicesImageXpress Micro Confocal (datasheet, Full Brochure) with live-cell, fluidics, transmitted light and confocal capabilities. We can now image 3D objects (resources) in an automated high-throughput fashion. We can image nearly any fluorophore with out collection of Semrockfilters. See below for our filter configurations.

Imagexpress Micro Confocal Filter User Manual Software

Please contact us for information on the IXMicro Confocal. For key card access to the IXMicro Confocal room (CCSR-0116) use iLabs.

iLabs IXM Confocal Schedule (click here to schedule training)
View only Calendar for non-registered users

Features and Capabilities of the ImageXpress Micro Confocal

Molecular Devices Imagexpress Micro Confocal

  • The IXMicro Confocal contains a fully automated inverted epifluorescence microscope -- User Guide
  • Capable of scanning a 6-, 24-, 48-, 96- or 384-well microplates, slides or any other microplate sized container.
  • Can recording multiple fluorescent images in a few minutes.
  • Can quantify morphological properties through the MetaXpress analysis software (Datasheet) -- Analysis Guide.
  • Our current ImageXpress Micro Confocal configuration permits the visualization of both fixed cells and live cells.
  • The instrument has a temperature-, humidity-, and carbon dioxide-controlled chamber.
  • Dual disk unit allowing for 3 imaging modes
  • A) 60 micron pinhole disk for basic confocal requirements
  • B) 50 micron slit for high-throughput confocal requirements
  • C) Widefield (non-confocal) imaging mode
  • Integrated Fluidics for imaging real time kinetic events, such as channel activation, providing for simultaneous dispense and imaging.
  • Can image non-fluorescent cells/objects with the transmitted light option
  • Fully integrated to an external automated incubator (ThermoCytomat 2C, 42 plates, Datasheet) to permit long-term or large-scale studies of cellular events via a robotic plate stacker (Precise Automation PF400 Microplate Handler, Datasheet, Green Button Go Automation Software, Datasheet, User Guide) thereby increasing the throughput of this workstation.
  • Training provided by HTBC staff sign up here; iLabs IXMicro Confocal Schedule
  • Two MetaXpress analysis computers Analysis Computer1, Analysis Computer2 also located in CCSR-0116
  • Prior to training, contact Jason Wu (x5-6004) to discuss what your imaging parameters.
DataSheet
Angiogenesis tube formationCell Cycle
Cell HealthCell Proliferation HT
Cell Scoring/multiwavelengthCount Nuclei
GranularityLive/Dead
MicronucleiMitotic Index
Monopole DetectionNeurite Outgrowth
TransfluorTransfluor HT
Translocation — basic, enhanced,Multi-wavelength, HT, Nuclear HT
3D Analysis OptionCurve fitting
Transmitted Light SegmentationAutoQuant Widefield 3D Deconvolution

Wells

Vendor

Catalog Number

Objectives

24

All

96

All

96

Corning/Costar*

All

384

All

384

Corning/Costar*

3712 or 4518 or 4581

All

*Secondary Preference

ObjectivesAura II Light EngineEmission FiltersDichroics
2X 0.1 Plan Apo, 8.5mm WD360_28nm*447_60nm409
4X 0.2 Plan Apo 20mm WD475_28nm*538_40nm506
10X 0.45 Plan Apo 4mm WD555_28nm*593_40nm562
20X 0.75 Plan Apo 1mm WD575_35nm*624_40nm593
40X 0.95 Plan Apo 0.25-0.16mm WD631_28nm*692_40nm660
60X 0.95 Plan Apo 0.21-0.11mm WD*Approximate still TBD
Oil Objectives
40X 1.3 Plan Fluor Oil 0.24mm WD
60X 1.4 Plan Apo 0.13 WD
100X 1.3 Plan Fluor 0.16mm WD
Label NameExcitationEmissionDichroic
DAPI360_28nm447_60nm409
FITC475_28nm538_40nm506
TRITC/Cy3555_28nm593_40nm562
TxRED575_35nm624_40nm593
Cy5631_28nm692_40nm660
FM 1-43475_28nm593_40nm562