Bactec Mgit 960 System User Manual

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BD BACTEC 460TB System.1,2 The BACTEC MGIT 960 instrument automatically directs the placement of each tube within the instrument and indicates positives with both a visual and an audible signal as they occur. The BACTEC MGIT960 System is nonradiometric. It uses MGIT media and patented sensors, making efficient use of advanced fluorometric. BBL MGIT™ System is the manual system while BACTEC MGIT 960 (MGIT 960) is the fully automatic system for detection of mycobacterial growth and drug susceptibility testing of. Johns hopkins. Study period were processed on MGIT 960 system for MTB culture and drug susceptibility testing (DST) fire is the emergence of MDR TB, which poses a major Lyophilized drugs (BACTEC MGIT 960 SIRE kit, Becton per standard procedure and were analyzed by using manual 2010/05, document number. Monitoring in the BACTEC MGIT 960 (Becton Dickinson) but the older manual system requires tubes to be examined for fl uorescence under a Wood’s lamp or with some other long wave UV light source. Though requiring more technical time, the manual system is a lot more affordable for laboratories with a smaller budget. In this study, the manual MGIT system (BBL MGIT). BACTEC™ Fluorescent Series User’s Manual 2. BD BACTEC™ 9000 Systems: A World of Difference in Blood Culture Page 3 07/2014 Figure 2 Fluorescent Technology and Sophisticated Algorithms: a Powerful Combination BMS DIAGNOSTICS (M) SDN BHD (485573-V) 19, Jalan 4/62A, Bandar Menjalara, Kepong, 52200 Kuala Lumpur, Malaysia.

Mycobacteria Growth Indicator Tube (MGIT) is intended for the detection and recovery of mycobacteria. The MGIT Mycobacteria Growth Indicator Tube contains 4 mL of modified Middlebrook 7H9 Broth base. The complete medium, with OADC enrichment and PANTA antibiotic mixture, is one of the most commonly used liquid media for the cultivation of mycobacteria.

All types of clinical specimens, pulmonary as well as extra-pulmonary (except blood and urine), can be processed for primary isolation in the MGIT tube using conventional methods. After processed specimen is inoculated, MGIT tube must be continuously monitored either manually or by automated instruments until positive or the end of the testing protocol.

  • 2Instruments

Principles of the Procedure[edit]

A fluorescent compound is embedded in silicone on the bottom of 16 × 100 mm round bottom tubes. The fluorescent compound is sensitive to the presence of oxygen dissolved in the broth. Initially, the large amount of dissolved oxygen quenches emissions from the compound and little fluorescence can be detected. Later, actively respiring microorganisms consume the oxygen and allow the fluorescence to be detected.

Tubes are filled with samples in the broth and continuously incubated at 37°C. The tubes are monitored for increasing fluorescence to determine if the tube is instrument positive; i.e., the test sample contains viable organisms. Fluorescence can be recorded by automated instruments such as Becton Dickinson's BACTEC MGIT 960 System, or manually using the BD BACTEC microMGIT fluorescence reader or a Wood's lamp or other long-wave UV light source.[1]

Instruments[edit]

Bactec Mgit 960 System User Manual

BACTEC MGIT 960 System[edit]

This instrument is produced by Becton Dickinson (BD). It is specially designed to accommodate Mycobacteria Growth Indicator Tube (MGIT) and incubate them at 37°C. The instrument scans the MGIT every 60 minutes for increased fluorescence. Analysis of the fluorescence is used to determine if the tube is instrument positive; i.e., the test sample contains viable organisms. An instrument-positive tube contains approximately 105 to 106 colony-forming units per milliliter (CFU/mL). Culture tubes which remain negative for a minimum of 42 days (up to 56 days) and which show no visible signs of positivity are removed from the instrument as negatives and discarded.[2] Its usefulness has been evaluated in the scientific literature.[3][4][5]

References[edit]

Bactec Mgit 960 System User Manual Enhanced

  1. ^Siddiqi, Salman H.; Sabine Rüsch-Gerdes (2006). Procedure Manual For BACTEC MGIT 960 TB System.
  2. ^Siddiqi, Salman H.; Sabine Rüsch-Gerdes (2006). Procedure Manual For BACTEC MGIT 960 TB System.
  3. ^'Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study' Enrico Tortoli, Paola Cichero, Claudio Piersimoni, M. Tullia Simonetti, Giampietro Gesu, and Domenico Nista Journal of Clinical Microbiology, November 1999, p. 3578-3582, Vol. 37, No. 11 [1]
  4. ^'Evaluation of Automated BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Four Major Antituberculous Drugs: Comparison with the Radiometric BACTEC 460TB Method and the Agar Plate Method of Proportion' Enrico Tortoli, Marta Benedetti, Alessandra Fontanelli, and M. Tullia Simonetti Journal of Clinical Microbiology, February 2002, p. 607-610, Vol. 40, No. 2 doi:10.1128/JCM.40.2.607-610.2002
  5. ^'Evaluation of the fully automated Bactec MGIT 960 system for the susceptibility testing of Mycobacterium tuberculosis to first-line drugs: a multicenter study' Fanourios Kontosa, Maria Maniatia, Christos Costopoulosb, Zoe Gittic, Stavroula Nicolaoub, Efymia Petinakia, Spyridoula Anagnostoub, Ioannis Tselentisc and Antonios N. Maniatis Journal of Microbiological Methods Volume 56, Issue 2, February 2004, Pages 291–294 doi:10.1016/j.mimet.2003.10.015
Retrieved from 'https://en.wikipedia.org/w/index.php?title=Mycobacteria_growth_indicator_tube&oldid=865812479#BACTEC_MGIT_960_System'

Mycobacteria Growth Indicator Tube (MGIT) is intended for the detection and recovery of mycobacteria. The MGIT Mycobacteria Growth Indicator Tube contains 4 mL of modified Middlebrook 7H9 Broth base. The complete medium, with OADC enrichment and PANTA antibiotic mixture, is one of the most commonly used liquid media for the cultivation of mycobacteria.

All types of clinical specimens, pulmonary as well as extra-pulmonary (except blood and urine), can be processed for primary isolation in the MGIT tube using conventional methods. After processed specimen is inoculated, MGIT tube must be continuously monitored either manually or by automated instruments until positive or the end of the testing protocol.

  • 2Instruments

Principles of the Procedure[edit]

Bactec Mgit 960 System User Manual

A fluorescent compound is embedded in silicone on the bottom of 16 × 100 mm round bottom tubes. The fluorescent compound is sensitive to the presence of oxygen dissolved in the broth. Initially, the large amount of dissolved oxygen quenches emissions from the compound and little fluorescence can be detected. Later, actively respiring microorganisms consume the oxygen and allow the fluorescence to be detected.

Tubes are filled with samples in the broth and continuously incubated at 37°C. The tubes are monitored for increasing fluorescence to determine if the tube is instrument positive; i.e., the test sample contains viable organisms. Fluorescence can be recorded by automated instruments such as Becton Dickinson's BACTEC MGIT 960 System, or manually using the BD BACTEC microMGIT fluorescence reader or a Wood's lamp or other long-wave UV light source.[1]

Instruments[edit]

BACTEC MGIT 960 System[edit]

This instrument is produced by Becton Dickinson (BD). It is specially designed to accommodate Mycobacteria Growth Indicator Tube (MGIT) and incubate them at 37°C. The instrument scans the MGIT every 60 minutes for increased fluorescence. Analysis of the fluorescence is used to determine if the tube is instrument positive; i.e., the test sample contains viable organisms. An instrument-positive tube contains approximately 105 to 106 colony-forming units per milliliter (CFU/mL). Culture tubes which remain negative for a minimum of 42 days (up to 56 days) and which show no visible signs of positivity are removed from the instrument as negatives and discarded.[2] Its usefulness has been evaluated in the scientific literature.[3][4][5]

References[edit]

Bactec Mgit 960 Package Insert

  1. ^Siddiqi, Salman H.; Sabine Rüsch-Gerdes (2006). Procedure Manual For BACTEC MGIT 960 TB System.
  2. ^Siddiqi, Salman H.; Sabine Rüsch-Gerdes (2006). Procedure Manual For BACTEC MGIT 960 TB System.
  3. ^'Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study' Enrico Tortoli, Paola Cichero, Claudio Piersimoni, M. Tullia Simonetti, Giampietro Gesu, and Domenico Nista Journal of Clinical Microbiology, November 1999, p. 3578-3582, Vol. 37, No. 11 [1]
  4. ^'Evaluation of Automated BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Four Major Antituberculous Drugs: Comparison with the Radiometric BACTEC 460TB Method and the Agar Plate Method of Proportion' Enrico Tortoli, Marta Benedetti, Alessandra Fontanelli, and M. Tullia Simonetti Journal of Clinical Microbiology, February 2002, p. 607-610, Vol. 40, No. 2 doi:10.1128/JCM.40.2.607-610.2002
  5. ^'Evaluation of the fully automated Bactec MGIT 960 system for the susceptibility testing of Mycobacterium tuberculosis to first-line drugs: a multicenter study' Fanourios Kontosa, Maria Maniatia, Christos Costopoulosb, Zoe Gittic, Stavroula Nicolaoub, Efymia Petinakia, Spyridoula Anagnostoub, Ioannis Tselentisc and Antonios N. Maniatis Journal of Microbiological Methods Volume 56, Issue 2, February 2004, Pages 291–294 doi:10.1016/j.mimet.2003.10.015

Bd Bactec Fx User Manual

Retrieved from 'https://en.wikipedia.org/w/index.php?title=Mycobacteria_growth_indicator_tube&oldid=865812479'